14A1 BM - IR Microscopy

Infrared Microspectroscopy ES

The TLS 14A IR beamline was designed to extract mid-infrared radiation for FTIR microspectroscopy studies, collecting 70 x 30 mrad2 (H x V) of the radiation generated by a bending magnet of Taiwan Light Source (TLS) storage ring of 1.5 GeV, covering spectral range of 4000-400 cm-1 (2.5 - 25.0 micron in wavelength). The highly collimated beam provides high lateral resolution and which is especially suitable for measurements on micron-sized samples.

The optics of the beamline consists of a plane mirror (M1), two polynomial mirrors (M2 and M3), and several steering and collimating mirrors (M4 and M5 are parabolic and plane mirrors, respectively.), designed by using SHADOW ray tracing simulation [1]. Mirror M1 is water-cooled and made of explosion-bonded Glidcop/ stainless-steel substrate, located 1.22 meters from the arc source of bending magnet. Mirror M1 is with a centered slot for transmitting the X-rays radiation trapped by a water-cooled beam dump, installed with its manipulator inside of chamber of the bending magnet. Mirrors M2 and M3 are high-order polynomial bendable to effectively and precisely focus the infrared radiation from extended arc source of the bending magnet [2-5] at the same location at the downstream beam path for the focused spot by applying stresses against the short-side of mirrors M2 and M3, respectively, to alter the focus length for each of M2 and M3 as illuminated in the Figure 1.

The end-station includes FTIR Spectrometer and Infrared microscope. The beamline is currently set up for multidisciplinary studies.

Figure 1. The optical layout of the NSRRC TLS 14A IR beamline.

Parameters of Light Source

Type1.23 Tesla Bending Magnet
Beam Spot Size< 15 micron (diffraction limited)
Energy Range4000-400 cm-1 (2.5-25.0 micron; 0.5-0.05 eV)
Brilliance (brightness)1 x 1016 (photons/sec/mm2/mrad2/200mA/0.1%BW)

Areas of Research

Material science [6-17], earth and environmental science [18-23], paleontology [24-26] and medical science [27-32]


  1. B. Lai and F. Cerrina, Nucl. Instr. and Meth., A246, 337 (1986).
  2. C.-I. Chen, Y.-C. Lo and K.-L. Tsang, “Optical Design and Construction of Mid-infrared beamline at SRRC”, SRRC/RBM/IM/99-06
  3. Y.-C. Lo, C.-I. Chen, C.-H. Chang, “Applications of synchrotron‐based Fourier transform infrared microspectroscopy technology”, Instruments Today, 126, 63-71 (2002)
  4. Y.-C. Lee, C.-I Chen, P.-Y. Huang, C.-Y Liu, “A study of Fourier-Transform infrared Microspectroscopy under Top-Up Injection Operation at NSRRC”, Instruments Today, 170, 7-15 (2009)
  5. Y.-C. Lee, P.-Y. Huang, Y.-C. Chang and C.-I. Chen, “An infrared kinetic study of wax physisorption for oral cavity cancer detection using synchrotron-based infrared microspectrscopy”, Instruments Today, 170, 47-60 (2009)
  6. M.Bahou, Y.-J. Wu, and Y.-P. Lee, “A New Method for Investigating Infrared Spectra of Protonated Benzene (C6H7+) and Cyclohexadienyl Radical (c-C6H7) Using Para-hydrogen”, J. Chem. Phys., 136, 154304 (2012). (IF:2.997)
  7. Y.-H. Wu, K.-C. Hsu, and C.-H. Lee, “Characterization of Fe-doped Lithium Aluminosilicate Glass-ceramic Materials by Synchrotron Radiation Techniques”, J. Ceram. Soc. Jpn., 120, 420 (2012). (IF: 1.108)
  8. H.-F. Chen and Y.-J. Wu*, “Structure, Stability, and Vibrational Fundamentals of Low-lying Isomers of C6H7+”, Comput. Theo. Chem., 1006, 100 (2013). (IF: 1.344)
  9. M.Bahou, Y.-J. Wu*, and Y.-P. Lee*, “Formation and Infrared Absorption of Protonated Naphthalenes (1-C10H9+ and 2-C10H9+) and Their Neutral Counterparts in Solid Para-hydrogen”, Phys. Chem. Chem. Phys. 15, 1907 (2013). (IF: 3.567)
  10. P.-Q. Yu, K. Theodoridou, H. Xin, P.-Y. Huang, Y.-C. Lee, B. R. Wood, J. Agric. Food Chem. 61, 7234(2013) (IF:2.906)
  11. L. Luo, J. Lv, C. Xu, and S. Zhang*, “Strategy for Characterization of Distribution and Associations of Organobromine Compounds in Soil Using Synchrotron Radiation Based Spectromicroscopies”, Anal. Chem. 86, 11002 (2014). (IF: 6.350)
  12. M. Tsuge*, M. Bahou, Y.-J. Wu, L. Allamandola, and Y.-P. Lee*, “Infrared Spectra of Ovalene (C32H14) and Hydrogenated Ovalene (C32H15) in Solid Para-hydrogen”, Phys. Chem. Chem. Phys. 18, 28864 (2016). (IF: 3.567)
  13. M.-H. Lin, S. Hy, C.-Y. Chen, J.-H. Cheng, J. Rick, N.-W. Pu, W.-N. Su, Y.-C. Lee and B.-J. Hwang*, “Resilient Yolk-Shell Silicon-Reduced Graphene Oxide/Amorphous Carbon Anode Material Achieved by a Synergistic Dual-Coating Process for Lithium-Ion Batteries”, ChemElectroChem 10.1002/celc.201600254 (2016). (IF: 3.506)
  14. W.-T. Chuang*, Y.-M. Hsu, E.-L. Lin, I.-M. Lin, Y.-S. Sun*, Y.-W. Chiang, C.-J. Su, Y.-C. Lee, and U.-S. Jeng, “Live Templates of a Supramolecular Block Copolymer for the Synthesis of Ordered Nanostructured TiO2 Films via Guest Exchange”, ACS Appl. Mater. Interfaces 8, 33221 (2016). (IF: 8.456)
  15. C.-W. Chen, W.-J. Syu, T.-C. Huang, Y.-C. Lee, J.-K. Hsiao, K.-Y. Huang, H.-P. Yu, M.-Y. Liao and P.-S. Lai*,” Encapsulation of Au/Fe3O4 nanoparticles into a polymer nanoarchitecture with combined near infrared-triggered chemo-photothermal therapy based on intracellular secondary protein understanding”, J. Mater. Chem. B, 5, 5774 (2017) (IF: 4.543)
  16. Y.-H. Hsieh, J.-H. Zhang, W.-C. Chuang, K.-H. Yu, X.-B. Huang, Y.-C. Lee, and C.-I. Lee* , “An in Vitro Study on the Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida Albicans”,Int. J. Mol. Sci. 19 , 337 (2018). (IF: 4.183)
  17. W.-J. Syu, C.-C. Huang, J.-K. Hsiao, Y.-C. Lee, Y.-T. Huang, P. Venkatesan, and P.-S. Lai*, “Co-precipitation Synthesis of Near-infrared Iron Oxide Nanocrystals on Magnetically Targeted Imaging and Photothermal Cancer Therapy via Photoablative Protein Denature”, Nanotheranostics 3, 236 (2019). (IF: 8.063)
  18. S.-E. Peng*, C.-S. Chen, Y.-F. Song, H.-T. Huang, P.-L. Jiang, W.-N. U. Chen, L.-S. Fang and Y.-C. Lee*, “Assessment of metabolic modulation in free-living versus endosymbiotic Symbiodinium using synchrotron radiation-based infrared microspectroscopy” Biol. Lett. 8(3), 434-437 (2012) (IF:3.651)
  19. A. H. M. S. Reza, J.-S. Jean, M.-K. Lee, T. R. Kulp, H.-F. Hsu,C.-C. Liu, Y.-C. Lee, “The binding nature of humic substances with arsenic in alluvial aquifers of Chianan Plain, southwestern Taiwan” Journal of Geochemical Exploration, 114,  98–108 (2012).(IF:1.312)
  20. C.-C. Liu, S. Kara, J.-S. Jean, C.-H. Wang, Y.-C. Lee, O. Sracek, Z. Li, J. Bundschuh, H.-J. Yang, C.-Y. Chen, “Linking geochemical processes in mud volcanoes with arsenic mobilization driven by organic matter”, Journal of Hazardous Materials, 262, 980– 988 (2013). (IF:4.553)
  21. A. Kunz*, B. A. Walther, L. Löwemark, and Y.-C. Lee, “Distribution and Quantity of Microplastic on Sandy Beaches along the Northern Coast of Taiwan”, Mar. Pollut. Bull. 111, 126 (2016). (IF: 3.782)
  22. J. Rathod, J.-S. Jean, W.-T. Jiang, I-H. Huang, B. H. Liu, Y.-C. Lee, Science of the Total Environment, 669, 527–539 (2019). (IF: 5.589)
  23. L. J. Bancina, B. A. Walther, Y. -C Lee, A. Kunz, “Two-dimensional distribution and abundance of micro- and mesoplastic pollution in the surface sediment of Xialiao Beach, New Taipei City, Taiwan”, Marine Pollution Bulletin 140, 75-85(2019), (IF: 3.782)
  24. R. R. Reisz*, T. D. Huang, E. M. Roberts, S. R. Peng, C. Sullivan, K. Stein, A. R. H. LeBlanc, D. Shieh, R. S. Chang, C. C. Chiang, C. Yang, and S. Zong, “Embryology of Early Jurassic Dinosaur from China with Evidence of Preserved Organic Remains”, Nature 496, 210 (2013). (IF: 43.070)
  25. k. S. Brink*, R. R. Reisz*, A. R. H. LeBlanc, R.-S. Chang, Y.-C. Lee, C.-C. Chiang, T. Huang, and D. C. Evans, “Developmental and evolutionary novelty in the serrated teeth of theropod dinosaurs”, Scientific Reports 5, Article number: 12338, (2015). (IF: 5.578)
  26. Y.-C. Lee*, C.-C. Chiang, P.-Y. Huang, C.-Y. Chung, T. D. Huang, C.-C. Wang, C.-I. Chen, R.-S. Chang, C.-H. Liao, R. R. Reisz*, “Evidence of preserved collagen in an Early Jurassic sauropodomorph dinosaur revealed by synchrotron FTIR microspectroscopy”, Nature Communications, 8, 14220, (2017). (IF: 12.124)
  27. H.-Y. Cheng, L.-J. Lai, and F.-H. Ko, “Rapid and Sensitive Detection of Rare Cancer Cells by the Coupling of Immunomagnetic Nanoparticle Separation with ELISA Analysis”, Int. J. Nanomed. 7, 2967 (2012). (IF: 4.471)
  28. L.-F. Chiu, P.-Y. Huang, W.-F. Chiang, T.-Y. Wong, S.-H. Lin, Y.-C. Lee*, D.-B. Shieh*, “Oral cancer diagnostics based on infrared spectral markers and wax physisorption kinetics”, Anal. Bioanal. Chem. 405(6), 1995-2007(2013) (IF:3.841)
  29. C.-K. Chen, M.-H. Chang, H.-T. Wu, Y.-C. Lee, T.-J. Yen, “Enhanced vibrational spectroscopy, intracellular refractive indexing for label-free biosensing and bioimaging by multiband plasmonic-antenna array”, Biosens. Bioelectron., 60, 343 (2014). (IF: 6.451)
  30. H.-Y. Cheng, F.-H. Ko, and L.-J. Lai*, “Using Novel Method to Detect Different Cancer-cell Stages of Model Human Lung Carcinoma”, J. Clin. Lab. Anal. 29, 285 (2015). (IF: 1.728)
  31. C.-H. Lee, C.-Y. Hsu, P.-Y. Huang, C.-I. Chen, Y.-C. Lee*, and H.-S. Yu*, “Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging”, Int. J. Mol. Sci., 17, 427 (2016). (IF: 3.257)
  32. Priprem, Y.-C. Lee, W. Limphirat, S. Tiyaworanant, K. Saodaeng, J. Chotitumnavee, N. Kowtragoon, “Eucalyptus ash alters secondary protein conformation of human grey hair and facilitates anthocyanin dyeing” PLoS ONE 13(7): e0199696 (2018) (IF: 2.806)

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+886-3-578-0281 Ext. 1141

BL Spokesperson

Lee, Yao-Chang (李耀昌)
+886-3-578-0281 Ext. 7333

BL Manager/Local Contact

Huang, Pei-Yu (黃佩瑜)
+886-3-578-0281 Ext. 7329


  • IR/Visible/UV/VUV Spectroscopy


  • Molecular science
  • Biomaterials
  • Biochemistry
  • Materials science
  • Chemistry
  • Polymer and soft matter
  • Bio-medical imaging

General Information

  • Source: 14A1
  • Energy Range: 0.5-0.05eV
  • Focused Spot Size: 10 x 13 μm²
  • Status: Operational

National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076