14A1 BM - IR Microscopy

Infrared Microspectroscopy ES

Synchrotron radiation-based infrared microspectroscopy (SR-IMS) beamline provides considerable brightness advantages over conventional IR sources. The combination of IMS with high brightness synchrotron radiation has greatly improved the lateral resolution of the technique by allowing high-quality FTIR spectra to be collected from samples and focused beam size is approaching the diffraction limit for the wavelength of mid-infrared radiation.
End-station of SR-IMS TLS14A1 has been open for public operation since September 2005. NSRRC SR-IMS is a technology of combination of the state-of-the-art FT-IR spectroscopy, optical microscope with all-reflected optics, and Infrared synchrotron radiation is generated and extracted from bending magnet edges in an electron storage ring of 1.5 GeV. IMS has the capability for identification and quantification of materials on a microscopic scale. As the shapes and forms of natural objects provided the foundation for biological science, FTIR spectroscopy has anchored our understanding of bio-molecular structure, such as protein secondary structures, spectral profile of nucleic acid and alteration in glycosylation of glycoconjugates. FTIR microscopic inspection reveals not only the fine details of tissue morphology but chemical components as well. With inventive skill and imagination, biological found ways to selectively stain tissue, visually defining chemical classes by specific colors. A stained tissue section is a vivid and detailed image of chemical distribution. Currently, IMS allows biologists to explore the complex chemistry of biological tissue directly and relate molecular chemistry with morphology. These analyses may probe composition at a single point or generation an extended map for interest of the sample area. Spectral or chemical images can be constructed from an array of points based on characteristic absorption of functional group using peak height, peak area, peak height ratio and peak area ratio for two peaks.
TLS 14A1 is the only infrared beamline in operation at NSRRC, some general information is listed here, and for more detailed information please visit the website of the Infrared Microspectroscopy Facility at NSRRC.content

Technical Information

Operational (general use)Yes

%Time available

(general use)

75-100 %
ExperimentsFTIR, ATR-FTIR and Infrared Microspectroscopy
Source    Arc extracted from Bending Magnet
Ring Current360 mA
Average Beam LifetimeInfinite Under NSRRC Top-Up Operation Conditions
Spectral Range4000-400 cm-1
Spectral Resolution0.125 cm-1
Spot Size  FWHM13 µm (H) x 10 µm (V)

Plan Mirror with center 1 mm slot (M1),  Bendable Horizontal Focusing Mirror (M2), Bendable Vertical Focusing Mirror (M3), 

and Parabolic Collimating Mirror (M4)

Acceptance Angle70 mrad (H) x 30 mrad (V)
Flux (photons/sec)  2 x 1012
Wavelength (Min.)2.5 µm
Wavelength (Max.)25.0 µm
MirrorsAu Coated
DetectorLN Cooled MCT/A and DTGS

OMNICTM 9.0, Atlµs,

and iPathologist (Beta version)

HardwareNicoletTM 6700 Spectrometer and Spectra Tech Continµm IR Microscope
Backup DevicesUSB 3.0 Portable Disk

Collaboration Team

Medical college Institute,

National Cheng Kung University


Dar-Bin Sheng, MD, Ph. D

(教授&醫師  謝達斌 博士)

Department of Pathology,

Kaohsiung Medical University Hospital


Hsin-Su Yu, MD, Ph. D     

(教授&醫師 余幸司 博士)

Chee-Yin Chai, MD, Ph. D

(教授&醫師 蔡志仁 博士) 

Yi-Ting Chen, MD,Ph. D

(醫師 陳怡庭 博士)

Hsinchu Mackay Memorial Hospital  


Shing-Jyh Chang, MD, Ph. D

(醫師 張幸治 博士 )

Tainan Chi Mei Medical Center


Wei-Fan Chiang, MD, Ph. D

(醫師 蔣維凡 博士)

Chang Gung Memorial Hospital,

Linkou Medical Center


Mei-Ching Yu, MD, Ph. D  

(醫師 余美靜 博士)

Chi-Nan Tseng, MD, Ph. D  

(醫師 曾棋南 博士)

Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine      



Chih-Hung Lee, MD, Ph. D

(教授&醫師 李志宏 博士)

Imperial College London,

Medicine school


Frederick Wai Keung Tam, MD, Ph.D

(教授&醫師 譚偉強 博士)

Find a Beamline

14A1 Home




Front End & Endstations

Training Course


Other BL Info

BL Schedule





+886-3-578-0281 Ext. 1141

BL Spokesperson

Lee, Yao-Chang (李耀昌)
+886-3-578-0281 Ext. 7333

BL Manager/Local Contact

Huang, Pei-Yu (黃佩瑜)
+886-3-578-0281 Ext. 7329


  • IR/Visible/UV/VUV Spectroscopy


  • Molecular science
  • Biomaterials
  • Biochemistry
  • Materials science
  • Chemistry
  • Polymer and soft matter
  • Bio-medical imaging

General Information

  • Source: BM
  • Energy Range: 0.5-0.05eV
  • Focused Spot Size: 13 x 10 μm²
  • Status: Operational

National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076